We offer four sealing films, each designed for a specific application:

• PCRSEAL-OA — Optical clear, pressure-sensitive adhesive (rim-seal only). Designed for qPCR / Real-Time PCR. Clean peel for downstream processing.
• PCRSEAL-OB — Optical clear, full-surface sticky adhesive. Designed for qPCR, especially 384-well plates and high-throughput screening.
• PCRSEAL-SF — Standard PCR sealing film for end-point PCR, sample transport, and general plate sealing.
• PCRSEAL-ALM — Aluminum foil adhesive seal for long-term storage, compound libraries, and applications requiring a vapor barrier down to −80 °C.

We also offer the PCRSEALer — a sealing film and capping tool designed for consistent, even pressure application.

Not sure which film you need? Try our Seal Finder.

Both are optically clear and designed for qPCR, but they differ in adhesive design:

• PCRSEAL-OA (Model A) uses a pressure-sensitive adhesive applied only around the well rims. The adhesive doesn't contact your sample, and the film peels cleanly for downstream processing. It uses a dual adhesive system (acrylic + silicone PSA) and activates with firm pressure.
• PCRSEAL-OB (Model B) has a full-surface sticky adhesive (acrylic). It provides maximum contact area for a strong hold, making it ideal for 384-well plates. The trade-off is that peeling is less clean than OA since adhesive covers the entire surface.

Both perform equivalently in qPCR fluorescence reads. Choose OA if you need clean peel; choose OB if you need maximum adhesion or are running 384-well plates.

Either PCRSEAL-OA or PCRSEAL-OB. Both are optically clear with consistent fluorescence transmission across the full excitation/emission spectrum used in SYBR Green, FAM, ROX, and multiplex assays.

Choose OA for 96-well plates or when you need clean peel for downstream processing. Choose OB for 384-well plates or when maximum adhesion is the priority.

Both films are validated on QuantStudio™, CFX96™, and LightCycler® platforms with equivalent Ct values.

PCRSEAL-ALM (Aluminum Seal). Its aluminum foil backing provides an impervious moisture and vapor barrier, and it's rated down to −80 °C — the widest temperature range in our lineup. It's pierceable for convenient sample retrieval without removing the entire seal. Ideal for compound libraries, DMSO-containing samples, and any application requiring long-term storage integrity.

No. All four PCRSeal films are adhesive-based and seal with hand pressure — no heat sealer or specialized equipment required. A sealing paddle, roller, or our PCRSEALer tool is recommended for even pressure distribution, but gloved hands work fine.

All PCRSeal films are compatible with standard 96-well and 384-well plates with raised rims. Film dimensions are approximately 142 × 79.3 mm, which fits all standard SBS-format microplates.

• PCRSEAL-OA: −80 °C to +120 °C
• PCRSEAL-OB: −80 °C to +120 °C
• PCRSEAL-SF: −80 °C to +110 °C
• PCRSEAL-ALM: −80 °C to +120 °C

Store at room temperature in a cool, dry place out of direct sunlight. Films are packaged 10 per sealed bag to minimize exposure and cross-contamination. When stored properly, films maintain performance for at least 5 years from the date of manufacture.

Our films are manufactured in a Class 100K cleanroom and each lot is tested for biological purity, PCR inhibitors, qPCR performance, and evaporation. They are certified PCR inhibitor-free, human DNA-free, DNase/RNase-free, ATP-free, and non-pyrogenic — tested per lot, not per process run.

Your lot-specific Certificate of Analysis documenting these results is always available on demand at pcrseal.com/coa.

PCRSEAL-OA is specifically designed for clean peel — since the adhesive is only around the well rims, the film lifts off cleanly for downstream processing (sequencing, cloning, etc.).

PCRSEAL-OB and PCRSEAL-SF are peelable but use full-surface adhesive, so removal is less clean.

PCRSEAL-ALM is not peelable — pierce the foil with a pipette tip to access individual wells.

The edge effect refers to increased evaporation from perimeter wells due to incomplete sealing at the plate borders. To prevent it, firmly press all four outside edges and corners of the film after sealing the center. Use a sealing tool or roller along each edge to ensure full contact. This is especially important for long thermal cycling protocols.

Allow at least 10 minutes at room temperature after sealing before beginning thermal cycling. This gives the adhesive time to bond fully, which improves seal integrity and reduces the risk of film lifting during rapid temperature transitions.

The PCRSEALer is a handheld sealing film and capping tool designed to apply consistent, even pressure across the plate. It's more ergonomic than a gloved finger and provides better pressure distribution than improvised tools. It also functions as an 8-strip cap remover.

View PCRSEALer product page →

Most major brands certify their manufacturing process (e.g., ISO certification of their facility). We go further — every production lot undergoes functional qPCR testing before release. This means the specific films you receive have been tested, not just the factory that made them.

Our films are manufactured in an ISO 9001:2015 and ISO 13485:2016 certified facility to PCRSeal OEM specifications.

Visit pcrseal.com/coa, enter your lot number (printed on the box label), and your CoA will be available immediately. No waiting for paperwork, no emails to chase — available 24/7 for audits, compliance, and verification.

Each CoA includes results for:

• Biological purity testing
• PCR inhibitor assays
• qPCR performance validation
• Evaporation QC

The CoA is specific to the production lot — not a generic document.

Our optical films have been independently tested head-to-head against Applied Biosystems® and Bio-Rad® films on QuantStudio™ 384-well and CFX96™ systems with equivalent Ct values and tighter replicate consistency. They are also compatible with LightCycler® and other standard Real-Time PCR platforms.

Yes. All PCRSeal films are manufactured in a facility certified under ISO 9001:2015 (quality management) and ISO 13485:2016 (medical device quality management) to PCRSeal OEM specifications. Films are produced in a Class 100K cleanroom.

PCRSEAL-OA is a functional replacement for:

• Applied Biosystems® 4311971
• Bio-Rad® Microseal® "C" (MSC1001)
• Excel Scientific™ ThermalSeal™ TSS-RTQ-100

PCRSEAL-OB is a functional replacement for:

• Applied Biosystems® 4306311
• Bio-Rad® Microseal® "B" (MSB1001)
• Excel Scientific™ ThermalSeal™ TS-RT2-100

PCRSEAL-SF is a functional replacement for:

• Applied Biosystems® AB0558
• Excel Scientific™ ThermalSeal™ 100-THER-PLT

PCRSEAL-ALM is a functional replacement for:

• Applied Biosystems® AB0626
• Bio-Rad® Microseal® "F" (MSF1001B)
• Excel Scientific™ AlumaSeal™ AF-100

PCRSeal films are designed as drop-in replacements for the films listed in our cross-reference tables. They fit all standard 96-well and 384-well plates with raised rims. Most labs can switch without formal revalidation. That said, if your protocol requires documented verification, we offer sample packs so you can validate on your own platform before committing.

PCRSeal films work with any thermocycler or Real-Time PCR instrument that uses standard SBS-format 96-well or 384-well plates. This includes Applied Biosystems QuantStudio™, Bio-Rad CFX96™ and CFX Opus, Roche LightCycler®, and all standard thermal cyclers. If your instrument uses standard microplates, our films will fit.

You can order directly through our website at pcrseal.com. Add products to your cart and check out with a credit card. For custom volume pricing, request a quote and we'll put together a package for you.

We accept all major credit cards (Visa, Mastercard, Amex, Discover) and Apple Pay / Google Pay. All orders are prepaid at checkout. For established accounts with consistent order history, invoicing terms may be available — request a quote to discuss.

Yes. Orders of 5 or more boxes of any single SKU receive a 5% discount plus free shipping, automatically applied at checkout. For larger institutional or distributor volume pricing, request a quote or visit our Partner With Us page.

Yes. Every film product has a sample pack option (10 films, $19.98) available directly on the product page — just select "Sample Pack" from the variant dropdown. Sample packs are limited to one per product per order. It's enough to validate performance on your platform before committing to a full box.

Yes. If you manage a lab stockroom, core facility, or institutional purchasing account, we offer dedicated pricing tiers based on volume and consistent lot supply. Request a quote or visit our Partner With Us page to start the conversation.

We currently ship within the contiguous United States. If you're located outside the US, contact us and we'll work with you on options.

Orders are processed quickly and shipped via FedEx Ground or USPS Priority Mail. Transit times vary by destination. You'll receive tracking information as soon as your order ships.

We ship via FedEx Ground and USPS Priority Mail, selected based on package weight and destination for the best combination of speed and value.

Shipping is a flat $8.95 per order. Orders of $375 or more ship free. Institutional and distributor accounts may have custom shipping arrangements.

We accept returns of unopened, undamaged product in original packaging within 30 days of delivery. Buyer pays return shipping. Refund is issued upon inspection. No returns on opened product unless defective. See our full Return & Refund Policy for details.

If you receive damaged or defective product, contact us with your order number and a photo of the issue. We'll replace it at no charge, including shipping. We stand behind our product quality.

Contact us with your order number and reason for return. We'll provide return instructions and a shipping address. Refunds are processed within 5–7 business days of receiving the returned product.

We're building a select network of life science distributors. If you serve research, clinical, or academic labs, visit our Partner With Us page and submit a distributor inquiry. We offer preferred and dealer tier pricing with volume incentives, marketing support, and CoA documentation on demand for your customers.

Yes. We offer volume pricing and consistent lot supply for high-throughput labs. Request a quote or visit our Partner With Us page to get started.

For individual evaluation, order a sample pack directly from any product page — just select "Sample Pack" from the variant dropdown (10 films, $19.98). For distributor evaluation samples, submit an inquiry through our Partner With Us page and we'll send product for your team to test.

We don't sell plates directly, but we partner with distributors who carry high-quality, affordable PCR and qPCR plates. Visit the PCR Plates tab on our Partner With Us page and we'll connect you with a trusted supplier.

Technical Data Sheets for each product are available on our Documentation page and linked from their respective product pages. They include dimensions, materials, adhesion specs, regulatory compliance information, and cross-reference data. If you can't find what you need, contact us and we'll send it directly.

Yes. Safety Data Sheets are available for all PCRSeal products on our Documentation page. All films are classified as non-hazardous with no special handling, storage, or disposal requirements beyond standard laboratory practices.

Yes. Our products carry a CE mark and comply with applicable EU regulations including IVDR (EU) 2017/746 (Class A), RoHS Directive 2011/65/EU, and REACH Regulation (EC) No. 1907/2006. EC Declaration of Conformity documentation is available on request — contact us.

PCRSeal products are labeled For Research Use Only. They are not intended for use in diagnostic procedures. If you have questions about your specific application, contact us.